How is the Elisa technique for detecting allergens in food?

Allergens are those substances that give way to allergic reactions, as a product of a response of the immune system to the appearance of foreign agents in the body. This reaction that occurs, does not happen in the same way in all people, occurs only with those sensitive to a specific antigen, which will make the immune system of the body captures them as dangerous or foreign, activating an inflammatory response to fight against them.

The immune system on contact with an allergen causes an exaggerated response that is evident in different organs of the human body. The allergen can come into contact with the body in various ways, for example, by ingesting or inhaling certain types of drugs or foods, or by contact with the skin, inducing serious symptoms such as rhinitis, sneezing, runny nose and even bronchial asthma.

Allergen screening uses a C-reactive protein test or immunochemical assays, such as the enzyme-linked immunosorbent assay, also known as the ELISA test, by its acronym. This test is a method of determining whether antibodies or foreign substances are present in a sample. The antibody is the protein produced by the immune system at the time antigens are detected.

Spectrophotometer and microplate reader

The spectrophotometer, is a equipment used in laboratories to measure the intensity of light that is absorbed when passing through a solution. This instrument is responsible for carrying out the ELISA test, measuring bacterial growth, DNA, RNA and protein analysis, among many other applications.

The microplate reader, on the other hand, is a type of spectrophotometer used mainly in the ELISA technique, it is characterized because it works in a limited range of wavelengths, between 400 and 750 nm, those necessary for the technique already mentioned. Works by detecting specific antigens or antibodies in a sample, the detected antigen will be immobilized in a solid phase because of antibodies, which directly or indirectly will produce a reaction, it will generate a producer that can be read in the spectrophotometer.

Kalstein spectrophotometer

At Kalstein we are MANUFACTURERS of laboratory and clinical equipment, our products have as main characteristic a high quality and technology. We have a wide variety of models at the most accessible PRICES you can find on the market. If you are considering PURCHASING a spectrophotometer, in our catalog you can find different models, for example, VIS UV spectrophotometer, grid, table and visible, as well as microplate readers.  To purchase one, just click on the following link HERE select the one of your preference and request the quote. We also remind you that at all times you will be advised by our experts. For information on our other products, see HERE